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KMID : 0620920090410090638
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Experimental & Molecular Medicine
2009 Volume.41 No. 9 p.638 ~ p.647
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Comprehensive analysis of microRNA-mRNA co-expression in circadian rhythm
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Na Young-Ji
Sung Jung-Hwan
Lee Suk-Chan
Lee Young-Ju
Choi Yeun-Joo
Park Woong-Yang
Shin Hee-Sup
Kim Ju-Han
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Abstract
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To investigate the potential role of microRNA (miRNA) in the regulation of circadian rhythm, we performed microarray- based expression profiling study of both miRNA and mRNA in mouse liver for 48 h at 4-hour intervals. Circadian miRNA-mRNA target pair is defined as the pair both elements of which show circadian expression patterns and the sequence-based target relationship of which can be predicted. Circadian initiators, Clock and Bmal1, showed inversely correlated circadian expression patterns against their corresponding miRNAs, miR-181d and miR-191, targeting them. In contrast, circadian suppressors, Per, Cry, CKIe and Rev-erba, exhibited positively correlated circadian expression patterns to their corresponding miRNAs. Genomic location analysis revealed that intronic region showed higher abundance of cyclic than non-cyclic miRNAs targeting circadian genes while other (i.e., 3’-UTR, exon and intergenic) regions showed no difference. It is suggested that miRNAs are involved in the regulation of peripheral circadian rhythm in mouse liver by modulating Clock:Bmal1 complex. Identifying specific miRNAs and their targets that are critically involved in circadian rhythm will provide a better understanding of the regulation of circadian- clock system.
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KEYWORD
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circadian rhythm, gene expression profiling, gene expression regulation, liver, mice, micro-RNA
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